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192 - welled "ARMS"

The 192-well MADGE system is designed to allow two arrays of 96 samples to be run on the same gel.

This is used for genotyping by ARMS (amplification refractory mutation system), with one reaction for each allele. The same angle of electrophoresis as 96-well MADGE is used, but track lengths are half.

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Click for software analysis of image

Madge 192 "ARMS"

2 x 96 sample, plus 2 x 12 control lanes outside the arrays

Wells volume 8ul

Run length 11mm

See the online protocols and articles from HUGO 2000 training meeting for more details and images of gels and analysis.

Easy identification of allelles can be seen following the software analysis of the original 192 welled MADGE gel on a FluorImager.

Link to Protocol

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MadgeBio thank Ian Day & his colleagues for images & protocols used in this website.