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Proteins
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Coomassie gel This Coomassie gel was run to show that you can get good resolution of proteins on a MADGE gel. Here we see a 15%, 37.5:1Poly Acrylamide, Madge gel running cell lysate they run true down the lanes and show banding - as you would see on a mini gel. The markers show excellent resolution of the markers, with resolution down to 6kD seen.
Coomassie stained 15% gel, 37.5:1 Bands are distinct in the marker lanes and the Lysates show banding Bands above 20kD are clearly resolved. Bands of a little as 6kD difference are resolved .
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Western Blotting We have carried out an number of Blotting experiments - including the analysis of tagged expressed proteins, and looking at time course experiments in Signal Transduction and Apoptosis, making use of the 2 Dimensional format of the gel (link to PCR Image) Traditionally Westerns have been carried out on mini gels (10 x 10 cm) and are limited to 8 - 12 samples per gel. Large experiments with 100s of samples in the cohorts have been time consuming or off putting. MADGE now allows you to analyse large numbers of samples easily AND you also have the 96 well microplate format that is the dominant collection and storage format in the Biotechnology market
Western Blot of Smad and B-Adaptin Showing clear resolution of the bands by ECL detection after blotting and probing of a Madge protein gel Dr Gao from Pharmingen Laboratories in San Diego said- "It would be easy to look at 3 or 4 bands if the proteins/ antibodies were carefully selected and the antibodies were of a good quality." |
This
PAGE IS CURRENTLY UNDER CONSTRUCTION!!
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Copyright
© 2001 MadgeBio Ltd | All Rights Reserved
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MadgeBio
thank Ian
Day & his colleagues for images & protocols used in this website.
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